NHS ester-PEG3-S-Methyl ethanethioate Of NHS ester PEG Is For
Targeted Drug Delivery
|Transparent and oil free liquid|
|-20℃ for Long Term, Keep cool and dry|
Important Product Information
• NHS ester-PEG3-S-Methyl ethanethioate is moisture-sensitive.
Store the vial of biotin reagent at 4°C with desiccant. To avoid
moisture condensation onto the product, equilibrate vial to room
temperature before opening.
• As directed in the procedure, dissolve the NHS ester reagent
immediately before use. The NHS moiety readily hydrolyzes and
becomes non-reactive; therefore, weigh and dissolve only a small
amount of the reagent at a time, and do not prepare stock solutions
for storage. Discard any unused reconstituted reagent.
• Avoid buffers containing primary amines (e.g., Tris or glycine)
as these will compete with the reaction. If necessary, dialyze or
desalt to exchange the protein sample into an amine-free buffer
such as phosphate-buffered saline (PBS).
• When Pegylating proteins in solution, excess non-reacted PEG
linker is easily removed by size exclusion using either desalting
columns or dialysis (Additional Information). A 10mL desalting
column is best suited for processing Pegylation reactions involving
1-10mg of protein in approximately 0.5-2mL. For smaller amounts of
protein and/or smaller reaction volumes, both the Pegylation
reaction and subsequent buffer exchange may be performed in a
single Thermo Scientific Slide-A-Lyzer MINI Dialysis Unit. For
larger reaction volumes than can be processed with a desalting
column, either split the sample between two columns.